<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Thomas, Paul M.</style></author><author><style face="normal" font="default" size="100%">Golly, Kouakou F.</style></author><author><style face="normal" font="default" size="100%">Virginia, Ross A.</style></author><author><style face="normal" font="default" size="100%">Zyskind, Judith W.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cloning of nod gene regions from mesquite rhizobia and bradyrhizobia and nucleotide sequence of the nodDgene from mesquite rhizobia</style></title></titles><keywords><keyword><style  face="normal" font="default" size="100%">JRN</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1995</style></year></dates><volume><style face="normal" font="default" size="100%">61</style></volume><pages><style face="normal" font="default" size="100%">3422-3429</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nitrogen-fixing symbiosis between acteria and the tree legume mesquite (&lt;i&gt;Prosopis glandulosa&lt;/i&gt;) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of &lt;i&gt;Rhizobium&lt;/i&gt; (mesquite) sp. strain HW17b and &lt;i&gt;Bradyrhizobium&lt;/i&gt; (mesquite) sp. strain HW10h and were shown to be closely linked.  All of the cosmid clones of rhizobia that extneded the host range of &lt;i&gt;Rhizobium&lt;/i&gt; (&lt;i&gt;Parasponia&lt;/i&gt;) sp. strain NGR234CS to mesquite also supported nodulatoin of a Sym&lt;sup&gt;-&lt;/sup&gt; mesquite strain. The cosmid clones of bradyrhizobia that extended the host range of &lt;i&gt;Rhizobium&lt;/i&gt; (&lt;i&gt;Parasponia&lt;/i&gt;) sp. strain NGR234CS to mesquite were only able to confer nodulation ability in the Sym&lt;sup&gt;-&lt;/sup&gt; mesquite strain if they also contained a &lt;i&gt;nodD&lt;/i&gt;-hybridizing region. Subclones containing just the &lt;i&gt;nodD&lt;/i&gt; genes of either genus did not extend the host range of &lt;i&gt;Rhizobium&lt;/i&gt; (&lt;i&gt;Parasponia&lt;/i&gt;) sp. to mesquite, indicating that the &lt;i&gt;nodD&lt;/i&gt; gene is insufficient for mesquite nodulation. The &lt;i&gt;nodD&lt;/i&gt; gene region is conserved among mesquite-nodulating rhizobia regardless of the soil depth from which they were collected, indicating descent from a common ancestor. In a tree of distance relationships. the NodD amino acid sequence from mesquite rhizobia clusters with homologs from symbionts that can infect both herbaceous and tree legumes, including &lt;i&gt;Rhizobium tropici&lt;/i&gt;, &lt;i&gt;Rhizobium leguminosarum&lt;/i&gt; bv. &lt;i&gt;phaseoli&lt;/i&gt;, &lt;i&gt;Rhizobium loti&lt;/i&gt;, and &lt;i&gt;Bradyrhizobium japonicum&lt;/i&gt;.</style></abstract><accession-num><style face="normal" font="default" size="100%">LTER.1995-91453</style></accession-num></record></records></xml>